Review



human brain pericytes  (PromoCell)


Bioz Verified Symbol PromoCell is a verified supplier
Bioz Manufacturer Symbol PromoCell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    PromoCell human brain pericytes
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Human Brain Pericytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/bio_rxiv__64898__2026__05__07__723127-224-19-4?v=PromoCell
    Average 98 stars, based on 1558 article reviews
    human brain pericytes - by Bioz Stars, 2026-07
    98/100 stars

    Images

    1) Product Images from "Endothelial YAP/TAZ rewiring under cardiometabolic stress drives sex-divergent vascular remodeling in heart failure with preserved ejection fraction"

    Article Title: Endothelial YAP/TAZ rewiring under cardiometabolic stress drives sex-divergent vascular remodeling in heart failure with preserved ejection fraction

    Journal: bioRxiv

    doi: 10.64898/2026.05.07.723127

    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain pericytes. ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Figure Legend Snippet: ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain pericytes. ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).

    Techniques Used: Immunofluorescence, Staining, Derivative Assay, Gene Expression, Expressing, Quantitative RT-PCR, Transfection



    Similar Products

    human brain pericytes  (PromoCell)
    98
    PromoCell human brain pericytes
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Human Brain Pericytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/bio_rxiv__64898__2026__05__07__723127-224-19-4?v=PromoCell
    Average 98 stars, based on 1 article reviews
    human brain pericytes - by Bioz Stars, 2026-07
    98/100 stars
    		  Buy from Supplier
    human brain vascular pericytes hvpcs  (Innoprot Inc)
    94
    Innoprot Inc human brain vascular pericytes hvpcs
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Human Brain Vascular Pericytes Hvpcs, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/10__1113_slash_jp291064-90-24-30?v=Innoprot+Inc
    Average 94 stars, based on 1 article reviews
    human brain vascular pericytes hvpcs - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    28041 human pericytes promocell  (PromoCell)
    95
    PromoCell 28041 human pericytes promocell
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    28041 Human Pericytes Promocell, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/pm41650956-905-89-92?v=PromoCell
    Average 95 stars, based on 1 article reviews
    28041 human pericytes promocell - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    human pericytes  (PromoCell)
    95
    PromoCell human pericytes
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Human Pericytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/pm41650956-926-2-7?v=PromoCell
    Average 95 stars, based on 1 article reviews
    human pericytes - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    c 12980 fluoroshieldtm  (PromoCell)
    95
    PromoCell c 12980 fluoroshieldtm
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    C 12980 Fluoroshieldtm, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/pm41650956-905-94-92?v=PromoCell
    Average 95 stars, based on 1 article reviews
    c 12980 fluoroshieldtm - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    innoprot p10363  (Innoprot Inc)
    94
    Innoprot Inc innoprot p10363
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Innoprot P10363, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/10__1002_slash_admt__202502614-284-4-4?v=Innoprot+Inc
    Average 94 stars, based on 1 article reviews
    innoprot p10363 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    placenta  (PromoCell)
    95
    PromoCell placenta
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Placenta, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/pmc13015779-230-26-28?v=PromoCell
    Average 95 stars, based on 1 article reviews
    placenta - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    vascular pericytes  (Innoprot Inc)
    94
    Innoprot Inc vascular pericytes
    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain <t>pericytes.</t> ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).
    Vascular Pericytes, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pericytes/pmc12818715-142-17-19?v=Innoprot+Inc
    Average 94 stars, based on 1 article reviews
    vascular pericytes - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain pericytes. ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).

    Journal: bioRxiv

    Article Title: Endothelial YAP/TAZ rewiring under cardiometabolic stress drives sex-divergent vascular remodeling in heart failure with preserved ejection fraction

    doi: 10.64898/2026.05.07.723127

    Figure Lengend Snippet: ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain pericytes. ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).

    Article Snippet: HUVECs from pooled donors (PromoCell; pooled from single-female donors: C-12200, 479Z024, 485Z034, 488Z021, 433Z035.1 ; male-pool: C-12203, 479Z016) and human brain pericytes (ScienCell, Reference: #1200, 27194, single donor) were cultured on 0.2% gelatine precoated dishes/flasks in EGM2-Bulletkit without antibiotics (Lonza, #CC-3156) or in pericyte medium (PM, ScienCell, #1201), respectively.

    Techniques: Immunofluorescence, Staining, Derivative Assay, Gene Expression, Expressing, Quantitative RT-PCR, Transfection